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Duty
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Description
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1
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Incubators
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-clean the incubators with 70%
EtOH, clean water pan and fill with sterile DI water + Bacdown; monitor CO2
level for incubators, change tanks when empty
-keep entire workspace on this
bench clean
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2
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Autoclave
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-autoclave “Dry cycle”: tips,
glasspipets, glassware, microcentrifuge tubes etc…
-autoclave “liquid cycle”: DI
water for incubators
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3
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Water bath
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-clean water bath weekly and make
sure water level is filled
- keep entire workspace on this
bench clean
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*4
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Media inventory
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-count media bottles and create
inventory log so when someone uses a bottle they must log it out and know how
much media is available at all times
* fill tip boxes, glasspipets, microcentrifuge tubes etc. for biohoods
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*5
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Fill carboys with MilliQ water
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-fill carboys with MilliQ water
- keep entire workspace on this
bench clean
* fill tip boxes, glasspipets, microcentrifuge tubes etc. for biohoods
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*6
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Cell inventory & LN2 tank
level monitoring/pH meter maintenance
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-momitor cell inventory and create
inventory log so when someone uses it they must log it out and know how much
cell tube is available at all times
-measure the level of LN2 and
record in log sheet, order LN2 when level is at “4”
-make sure pH probe is stored in
electrode solution
* fill tip boxes, glasspipets, microcentrifuge tubes etc. for biohoods
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7
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Aliquot reagents
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-Aliquot HBSS, Try-EDTA, Trypsin,
and TNS
-make sure entire stock is always
available
-prepare buffers that are
routinely stocked
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8
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Biohoods/Fume hoods (hazardous
waste)
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-remove everything from inside,
remove lower platform and clean under it. Replace lower platform and clean
the hood thoroughly
-check the waste collection bottle
at the floor, and make sure biohazard waste bottle is no more then ¾ full. If
full, pore them in a pan, add bleach, wait ~30min, dump into the sink with
running water
-label the hazardous waste
properly and complete electronic request forms to pick up (usually every
Wed.)
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9
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Biohazard bin/sharps containers
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- biohazardous bin are no more
than ¾ full, objects are not exposed, and lid is on
-put the sharps containers in the
big biohazardous bin if they are full
-take biohazard bags to autoclave
room
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*10
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Clean area (room 3444)
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-keep area around microwave clean,
clean food fridge and microwave, recycle papers
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*11
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Dishes
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-wash dishes (dish washer is now
available!)
-after dishes are clean and dry,
prepare them to be autoclaved (if needed)
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12
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RNA/Protein Area
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-maintain and clean area for
RNA/protein (RT-PCR and Westerns) analysis including new bench covers and
maintenance of equipment.
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1
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Keep
workspace clean
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-clean
your workspace once you done/before you leave
-if
needed, change counter absorbent pads, wipe up spills, chemicals, etc.
-put the
equipment back when finished (i.e. western blot equipment)
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2
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tips
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-Fill 3
different boxes of tips (1000, 200, and 10 microliter) per week to use
outside the biohoods
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3
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Supply
shortage report
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-make a
note in a board when you notice any supply’s run out such as tips,
glasspipets, centrifuge tubes, microcentrifuge tubes, and etc.
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4
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deliveries
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-put
delivered items away; make sure items are stored at indicated temperatures,
and check packing slip to items received
-put
packing slip in a packing slip collection box
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5
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Refrigerator/freezer
maintenance (maybe once a month)
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-keep
fridge/freezer organized
-inventory
general use items
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